LOCUS       NEU2-pGEc2              6351 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(5829..5958)
                     /note="3'-ITR"
     misc_feature    complement(5527..5741)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    4979..5520
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4158..4180
                     /note="attB2"
     misc_feature    4958..4963
                     /note="BstB1 site"
     misc_feature    4181..4876
                     /note="Super GFP"
     misc_feature    4877..4927
                     /note="AviTag"
     misc_feature    4928..4951
                     /note="His Tag"
     PCR_frame       3011..4157
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..4135
                     /note="OPTIMIZED NEU2 coding region"
     misc_feature    4136..4157
                     /note="TEV"
     vector          join(4166..6351,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4165
                     /source="NEU2 pDONR"
                     /type="Entry Clone"
     source          1..6351
                     /dnas_title="NEU2-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGCTTCTC TGCCTGTGCT GCAGAAAGAA AGCGTGTTCC AGTCTGGCGC
     3061 CCACGCCTAT AGAATCCCTG CTCTGCTGTA TCTGCCTGGC CAGCAGTCTC TGCTGGCTTT
     3121 TGCCGAGCAG AGAGCCAGCA AGAAGGACGA GCACGCCGAG CTGATTGTGC TGAGAAGAGG
     3181 CGATTACGAC GCCCCTACAC ATCAGGTGCA GTGGCAGGCT CAGGAAGTGG TCGCTCAGGC
     3241 TAGACTGGAT GGCCACAGAT CCATGAACCC CTGCCCTCTG TATGATGCCC AGACCGGCAC
     3301 CCTGTTTCTG TTCTTTATCG CCATCCCCGG CCAGGTCACA GAACAGCAGC AGCTGCAGAC
     3361 CAGAGCCAAC GTGACAAGAC TGTGTCAGGT CACCAGCACC GACCACGGCA GAACATGGTC
     3421 TAGCCCCAGA GATCTGACAG ATGCCGCCAT CGGCCCTGCC TACAGAGAGT GGTCTACATT
     3481 TGCCGTGGGC CCTGGACACT GTCTCCAGCT GCATGATAGA GCCAGATCAC TGGTGGTGCC
     3541 TGCCTATGCC TATCGGAAGC TGCACCCTAT CCAGAGGCCT ATCCCTAGCG CCTTCTGCTT
     3601 TCTGTCTCAC GATCACGGAA GAACCTGGGC CAGAGGACAC TTCGTGGCAC AGGATACACT
     3661 GGAATGCCAG GTGGCCGAGG TGGAAACAGG CGAACAGAGG GTCGTGACAC TGAACGCCAG
     3721 ATCTCACCTG AGAGCTAGAG TGCAGGCCCA GAGCACCAAC GATGGCCTGG ATTTTCAGGA
     3781 ATCCCAGCTG GTCAAGAAAC TGGTGGAACC TCCTCCACAG GGCTGTCAGG GCTCTGTGAT
     3841 TAGCTTCCCT AGCCCTAGAA GCGGACCTGG ATCTCCTGCT CAGTGGCTGC TGTACACACA
     3901 CCCAACACAC AGCTGGCAGA GGGCTGATCT GGGCGCCTAT CTGAATCCTA GACCTCCTGC
     3961 ACCAGAGGCT TGGAGCGAAC CTGTGCTCCT GGCCAAGGGC AGCTGTGCCT ATAGCGATCT
     4021 GCAGTCTATG GGCACCGGAC CTGATGGCTC TCCACTGTTT GGATGCCTGT ACGAGGCCAA
     4081 CGACTACGAA GAGATCGTGT TCCTGATGTT CACCCTGAAG CAGGCCTTCC CTGCCgaaaa
     4141 cttgtacttt caaggctCCA GCTTTCTTGT ACAAAGTGGT ATGAGCAAGG GCGAGGAACT
     4201 GTTCACCGGC GTGGTGCCCA TCCTGGTGGA ACTGGATGGC GACGTGAACG GCCACAAGTT
     4261 CTCTGTGCGG GGAGAGGGCG AAGGCGACGC CACAAATGGC AAGCTGACCC TGAAGTTCAT
     4321 CTGCACCACC GGCAAGCTGC CCGTGCCTTG GCCTACCCTC GTGACCACAC TGACCTACGG
     4381 CGTGCAGTGC TTCAGCAGAT ACCCCGACCA TATGAAGCGG CACGACTTCT TCAAGAGCGC
     4441 CATGCCCGAG GGCTACGTGC AGGAACGGAC CATCAGCTTC AAGGACGACG GCACCTACAA
     4501 GACCAGAGCC GAAGTGAAGT TCGAGGGCGA CACCCTCGTG AACCGGATCG AGCTGAAGGG
     4561 CATCGATTTC AAAGAGGACG GCAACATCCT GGGCCACAAG CTGGAGTACA ACTTCAACAG
     4621 CCACAACGTG TACATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT
     4681 CCGGCACAAC GTGGAAGATG GCAGCGTGCA GCTGGCCGAC CACTACCAGC AGAACACCCC
     4741 CATCGGAGAT GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCGTGCT
     4801 GAGCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTCG TGACCGCCGC
     4861 TGGCATCACC CACGGCATGA GCGGCCTGAA CGACATCTTC GAGGCCCAGA AAATCGAGTG
     4921 GCACGAGCAC CACCATCATC ACCATCACCA CTGATGATTC GAAAAATAAT AAGGATCCAA
     4981 TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC
     5041 TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT
     5101 GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG
     5161 GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG
     5221 TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT
     5281 TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT
     5341 GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC
     5401 CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA
     5461 TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG
     5521 AGATCTGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG
     5581 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT
     5641 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC
     5701 AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ACTCGAGTTA AGGGCGAATT
     5761 GCCGATTAGG ATCTTCCTAG AGCATGGCTA CGTAGATAAG TAGCATGGCG GGTTAATCAT
     5821 TAACTACAAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT
     5881 CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT
     5941 GAGCGAGCGA GCGCGCAGCC TTAATTAAGA GCTCATGGCG CGCCTAGGCC TTGACGGCCT
     6001 TCCGCCAATT CGCCCTATAG TGAGTCGTAT TACGTCGCGC TCACTGGCCG TCGTTTTACA
     6061 ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC
     6121 TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAA ACGCCCTTCC CAACAGTTGC
     6181 GCAGCCTGAA TGGCGAATGG GAGCGCCCTG TAGCGGCCAC TCAACCCTAT CTCGGTCTAT
     6241 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT
     6301 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA G
//

